Defensive Responses of Rice Genotypes for Resistance Against Rice Leaffolder Cnaphalocrocis medinalis

doi:10.1016/S1672-6308(13)60149-3

Abstract

Abstract:

The experiment was carried out to assess the reaction of different categories of rice genotypes viz., resistant, susceptible, hybrid, scented, popular and wild in response to the infestation by rice leaffolder (RLF), Cnaphalocrocis medinalis (Guenee) and to explore the possible use of these genotypes in developing RLF-resistant rice varieties. The changes of various biochemical constituents such as leaf soluble protein, phenol, ortho-dihydroxy phenol, tannin and enzymes viz., peroxidase, phenyl alanine ammonia lyase (PAL) were assessed spectrophotometrically in all the rice genotypes before and after RLF infestation. The protein profile was analyzed using sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDS-PAGE) method. A significant constituent of biochemical content such as tannin, phenol and ortho-dihydroxy phenol has been increased along with enzyme activities of peroxidase and PAL in the infested resistant (Ptb 33, TKM6 and LFR831311) and wild rice genotypes (Oryza minuta and O. rhizomatis). A decrease in leaf protein content was evident invariably in all the infested rice genotypes. It is also evident that the contents of biochemicals such as phenol, ortho-dihydroxy phenol and tannin were negatively correlated with leaffolder damage. However, leaf protein content was positively correlated with the damage by rice leaffolder. SDS-PAGE analysis for total protein profiling of healthy and C. medinalis-infested genotypes revealed the enhanced expression of a high molecular weight (> 97 kDa) protein in all the genotypes. Besides, there was also an increased induction of a 38 kDa protein in C. medinalis infested resistant genotypes, which was absent in uninfested plants. The present investigation proved that the elevated levels of biochemicals and enzymes may play a vital role in rice plants resistance to RLF.

Key words: rice genotype, biochemical, defense enzyme, rice leaffolder, resistance

M. PUNITHAVALLI, N. M. MUTHUKRISHNAN, M. BALAJI RAJKUMAR. Defensive Responses of Rice Genotypes for Resistance Against Rice Leaffolder Cnaphalocrocis medinalis[J]. RICE SCIENCE, 2013, 20(5): 363-370.

References

Baldwin I T. 1999. The eco-physiological complexity of plant responses to insect herbivores. Planta, 208: 137–145.
Beck D L, Dunn G M, Routley D O, Bowman J S. 1983. Biochemical basis of resistance in corn to the corn leaf aphid. Crop Sci, 23: 995–998.
Bharathi M. 1996. Biochemical basis of resistance in rice to insect pests. In: Ananthakrishnan T N. National Symposium on Biochemical Bases of Host Plant Resistance of Insects. New Delhi: Indian Agricultural Research Institute, National Academy of Agricultural Sciences: 26–34.
Bwins R E. 1971. Methods for estimation of tannin in grain sorghum and other food stuff. Agron J, 63: 511–512.
Cahill D M, Mc Comb J A. 1992. A comparison of changes in phenylalanine lyase activity, lignin and phenolic synthesis in the roots of Eucalyptus calophylla (field resistant) and E. marginata (susceptible) when infected with Phytopthora cinnamonii. Physiol Mol Plant Pathol, 46: 315–332.
Edwards P J, Wratten S D. 1983. Wound induced defenses in plants and their consequences for patterns of insect grazing. Oecologia, 59: 88–93.
Felton G W, Donato K K, Broadway R M, Duffey S S. 1992. Impact of oxidized plant phenolics on the nutritional quality of dietary protein to a noctuid herbivore, Spodoptera exigua. J Insect Physiol, 38(4): 277–285.
Gasper T, Panel L, Thrpe T, Grepping H. 1982. Peroxidases: A Survey of Their Biochemical and Physiological Roles in Higher Plants. Geneva: University of Geneva Press: 1–20.
Grayer R J, Kimmins F M, Stevenson P C, Wijayagunasekara H N P. 1994. Phenolics in rice phloem sap as sucking deterrents to the brown planthopper, Nilaparvata lugens Stal. Acta Hort, 381: 691–694.
Haukioja E, Niemela P. 1977. Retarded growth of a geometrid larva after mechanical damage to leaves of its host tree. Ann Zool Fenn, 14: 48–52.
Hori K. 1973. Studies on feeding habits of Lygus disponsi Lannavuori (Hemiptera: Miridae) and the injury to host plant: III. Phenolic compounds, acid phosphatase and oxidative enzymes in the injured tissue of sugar beet leaf. Appl Ent Zool, 8: 103–112.
Hori K, Atalay R. 1980. Biochemical changes in the tissue of Chinese cabbage injured by the bug Lygus disponsi. Appl Ent Zool, 15: 234–241.
Johnson G, Scheal A L. 1957. Relation of chlorogenic acid to scab resistance in potatoes. Science, 115: 627–629.
Johnson R, Narvaez J, Ryan C. 1989. Expression of proteinase inhibitors I and II in transgenic tobacco plants: Effects on natural defense against Manduca sexta larvae. Proc Natl Acad Sci USA, 86: 9871–9875.
Jones D H. 1984. PAL regulation of its induction and its role in plant development. Phytochemistry, 23: 1346–1359.
Jyothsna Y, Kapil M, Usha Rani P. 2009. Effects of herbivore feeding on biochemical and nutrient profile of castor bean, Ricinus communis L. plants. Allelopathy J, 24(1): 131–142.
Khan Z R, Rueda B P, Caballero P. 1989. Behavioural and physiological responses of rice leaffolder, Cnaphalocrocis medinalis to selected wild rices. Entomol Exp Appl, 52: 7–13.
Kogan M, Paxton J. 1983. Natural inducers of plant resistance to insects. In: Hedin P A. Plant Resistance to Insects. American Chemical Society Symposium. Series, 208. Washington DC: American Chemical Society: 153–171.
Kushwaha K S, Singh R. 1984. Leaffolder (LF) outbreak in Haryana. Int Rice Res Newsl, 9(6): 20.
Lagrimini L M, Rothstein S. 1987. Tissue specificity of tobacco peroxidase enzymes and their induction by wounding and TMV infection. Plant Physiol, 84: 438–442.
Lowry O H, Rosebrough N J, Farr A L, Randall R J. 1951. Protein measurement with folin-phenol reagent. J Biol Chem, 193: 268–275.
Malick C P, Singh M B. 1980. Plant Enzymology and Histo- enzymology: A Text Manual. New Delhi: Kalyani Publishers: 286.
Mohan S, Purushothaman D, Jayaraj S, Rangarajan A V. 1988. PAL-ase activity in roots of Sorghum bicolor (L.) inoculated with Azospirillum. Curr Sci, 57: 492–493.
Nadarajan L, Nair N R. 1983. Screening of leaffolder resistance. Int Rice Res Newsl, 8(3): 5.
Radja Commare R, Nandakumar R, Kandan A, Suresh S, Bharathi M, Raguchander T, Samiyappan R. 2002. Pseudomonas fluorescences based bio-formulation for the management of sheath blight and leaffolder insect in rice. Crop Prot, 21: 671–677.
Raghumoorthy K N, Gunathilagaraj K. 1988. Field incidence of and host resistance to Angoumois grain moth (AGM). Int Rice Res Newsl, 13(4): 12.
Rajendran R, Rajendran B, Sundara Babu P C. 1986. Varietal resistance of rice leaffolder to Cnaphalocrocis medinalis. Int Rice Res Newsl, 11: 17–18.
Rath L K, Mishra D S. 1998. Biochemical basis of resistance in rice to white backed planthopper, Sogatella furcifera (Horvath). Environ Ecol, 16: 361–364.
Rekha, Ram L, Singh R, Singh R. 2001. Sources and mechanisms of resistance in rice against rice leaffolder Cnaphalocrocis medinaus (Guenee): A review. Agric Rev, 22(1): 1–12.
Sadasivam S, Manickam A. 1996. Biochemical Methods. Coimbatore: New Age International Limited and Tamil Nadu Agricultural University: 246.
Saxena R C. 1986. Biochemical bases of resistance in rice varieties. In: Green M B, Hedin P A. Natural Resistance of Plants to Pests: Role of Allelochemicals. Washington: American Chemical Society: 142–159.
Shrivastava S K. 1989. Leaffolder (LF) damage and yield loss on some of the selected rice varieties. Int Rice Res Newsl, 14(5): 10.
Sinha S, Balasaraswathi R, Selvaraju K, Shanmugasundaram P. 2005. Molecular and biochemical markers associated with leaffolder (Cnaphalocrocis medinalis G.) resistance in rice (Oryza sativa L.). Ind J Biochem Biophys, 42: 228–232.
Southerton S G. 1990. Changes in phenylalanine ammonia lyase and peroxidase activities in wheat cultivars expressing resistance to the leaf rust fungus. Plant Physiol, 39: 223–230.
Thayumanavan B, Velusamy R, Sadasivam S. 1990. Phenolic compounds, reducing sugars and free amino acids in rice leaves of varieties resistant to thrips. Int Rice Res Newsl, 11(1): 14–15.
Usha Rani P, Jyothsna Y. 2009. Physiological changes in the groundnut, Arachis hypogaea L. plant due to the infection of a fungal pathogen, Cercosporidium personatum Deighton. Allelopathy J, 23(2): 369–378.
Vidyachandra B, Roy J K, Das B. 1981. Chemical differences in rice varieties susceptible or resistant to gall midge and stem borer. Int Rice Res Newsl, 6: 7–8.
Waldbauer G P, Marciano A P. 1979. Rice Leaf Folder: Mass Rearing and a Proposal for Screening for Varietal Resistance in the Greenhouse. Manila, Philippines: International Rice Research Institute: 1–8.

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